Main techniques used in the laboratory
Techniques involve primarily: cell cultures, cell cycle synchronization, replication and transcription essays, RNAi, gene knock-outs, immunolabelling coupled with light (fluorescent, confocal) and advanced electron microscopy, protein immunoprecipitation and purification from cellular extracts, 2D electrophoresis, Western blotting, microinjections into living cells, molecular cloning, GFP technology, cell transfections, DNA/RNA hybridization in situ/on blots, production of antibodies, digital imaging, computer-assisted 3D reconstructions, image processing, stereology, production of monoclonal antibodies, knock-out mice.

                                                              The Laboratory

                                   Sample Preparation for Electron Microscopy

          LEICA EMPACT2: high-pressure freezing of samples
                     preventing  the formation of crystalic ice

         FEI VITROBOT: freezing of
             samples in thin layers

                        LEICA EM FSP: replacement of ice in the
                                           sample with resins

                  LEICA EM UltraCut6: cutting ultrathin
                                 (cryo)sections 50 - 100 nm
                                                           Electron Microscopy

           FEI TECNAI: a top research 200kV transmission
                  microscope with
tomography, cryostage
                       and elemental mapping (EELS, GIF)

   FEI MORGAGNI: a routine 100kV 
                  transmission EM
                                                            Light Microscopy

                  LEICA DM 6000: research grade fluorescence
                                        upright microscope


                LEICA DMI 6000: research grade fluorescence
inverted microscope for observation of
                                                 living cells


           NICON TE 300 ECLIPSE: inverted fluorescence
for microinjections into living cells