Main techniques used in the laboratory

Techniques involve primarily: cell cultures, cell cycle synchronization, replication and transcription essays, RNAi, gene knock-outs, immunolabelling coupled with light (fluorescent, confocal) and advanced electron microscopy, protein immunoprecipitation and purification from cellular extracts, 2D electrophoresis, Western blotting, microinjections into living cells, molecular cloning, GFP technology, cell transfections, DNA/RNA hybridization in situ/on blots, production of antibodies, digital imaging, computer-assisted 3D reconstructions, image processing, stereology, production of monoclonal antibodies, knock-out mice.

 

The Laboratory
Sample Preparation for Electron Microscopy
LEICA EMPACT2: high-pressure freezing of samples                      preventing  the formation of crystalic ice	FEI VITROBOT: freezing of              samples in thin layers
LEICA EM FSP: replacement of ice in the                                            sample with resins	LEICA EM UltraCut6: cutting ultrathin                                  (cryo)sections 50 - 100 nm
Electron Microscopy
FEI TECNAI: a top research 200kV transmission                   microscope with tomography, cryostage                        and elemental mapping (EELS, GIF)	FEI MORGAGNI: a routine 100kV                    transmission EM
Light Microscopy
LEICA DM 6000: research grade fluorescence                                         upright microscope	LEICA DMI 6000: research grade fluorescence                        inverted microscope for observation of                                                  living cells
NICON TE 300 ECLIPSE: inverted fluorescence                microscope for microinjections into living cells