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Program

 

Monday

 

12:00 – 13:00

Registration

 

13:00 – 13:30

Introduction and short presentations of the participants

Pavel Hozák

13:30 – 14:45

Basics of TEM - Theory
General TEM construction
The parts, filament (Wehnelt), apertures, lenses, Cs, Cc and focal length, high tension.

Wim Busing

14:45 – 17:00

Basics of TEM - Theory (continued)
Alignments, (recognizing mis-alignments), total alignment, TEM imaging bright/dark field, resolution, in-focus, over andunder focus, astigmatism (condenser, objective and diffraction astigmatism), contrast. Digital cameras.

Matthijn Vos

 

Tuesday

 

09:00 – 12:00

Practical session: Alignments
- Morgagni
- CM 100
- T20
Direct alignments, alignments procedures, test specimens.

Wim Busing
Old°ich Benada
Matthijn Vos

12:00 – 13:00

Lunch

 

13:00 – 17:00

Practical session: TEM imaging
- Morgagni
- CM 100
- T20
Bright/dark field, resolution, focus, astigmatism, contrast.

Wim Busing
Old°ich Benada
Matthijn Vos

 

Wednesday

 

09:00 – 10:45

Theory: How to choose a microscope for your applications?
Pro/con Tecnai, CM 100 and Morgagni and other tools if one can choose?
Special techniques, cryo, tomo, filtered imaging.

Wim Busing
Matthijn Vos

10:45 – 11:15

Break

 

11:15 – 12:00

Practical session: Catch-up alignments “special” techniques in short

Wim Busing

12:00 – 13:00

Lunch

 

13:00 – 16:00

Practical session: continued

Matthijn Vos

16:00 – 17:00

Theory: Filter technology
Post – versus in - column filters, types of microscopes, application flexibility versus resolution.

Wim Busing

 

Thursday

 

09:00 – 12:00

Practical session: Repetition of different parts
- Morgagni
- CM 100
- T20

Vlada Philimonenko
Old°ich Benada
Matthijn Vos

12:00 – 13:00

Lunch

 

13:00 – 17:00

Practical session (continued)
- Morgagni
- CM 100
- T20

Vlada Philimonenko
Old°ich Benada
Matthijn Vos

18:00 – ?

Evening party - FEI sponsored

 

 

Friday

 

09:00 – 10:00

Theory: The best resolution and instrument requirements
The highest resolution in Life Sciences, best practice in biology.

Matthijn Vos

10:00 – 11:00

Theory: Sample preparation and cryo-electron microscopy; future prospects of TEM in biomedicine

Pavel Hozák

11:00 – 11:30

Short demonstration of sample preparation

Vlada Philimonenko

11:30 – 12:00

Evaluation of the workshop

Pavel Hozák

12:00

Lunch, departure

 

TEM in Life Sciences 2013 Program (.pdf)