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Monday |
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12:00 – 13:00 |
Registration |
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13:00 – 13:30 |
Introduction and short presentations of the participants |
Pavel Hozák |
13:30 – 14:45 |
Basics of TEM - Theory General TEM construction
The parts, filament (Wehnelt), apertures, lenses, Cs, Cc and focal length, high tension. |
Wim Busing |
14:45 – 17:00 |
Basics of TEM - Theory (continued)
Alignments, (recognizing mis-alignments), total alignment, TEM imaging bright/dark field, resolution, in-focus, over andunder focus, astigmatism (condenser, objective and diffraction astigmatism), contrast. Digital cameras.
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Matthijn Vos |
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Tuesday |
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09:00 – 12:00 |
Practical session: Alignments
- Morgagni
- CM 100
- T20
Direct alignments, alignments procedures, test specimens. |
Wim Busing
Oldøich Benada
Matthijn Vos |
12:00 – 13:00 |
Lunch |
|
13:00 – 17:00 |
Practical session: TEM imaging
- Morgagni
- CM 100
- T20
Bright/dark field, resolution, focus, astigmatism, contrast. |
Wim Busing
Oldøich Benada
Matthijn Vos |
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Wednesday |
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09:00 – 10:45 |
Theory: How to choose a microscope for your applications?
Pro/con Tecnai, CM 100 and Morgagni and other tools if one can choose?
Special techniques, cryo, tomo, filtered imaging. |
Wim Busing
Matthijn Vos |
10:45 – 11:15 |
Break |
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11:15 – 12:00 |
Practical session: Catch-up alignments “special” techniques in short |
Wim Busing |
12:00 – 13:00 |
Lunch |
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13:00 – 16:00 |
Practical session: continued |
Matthijn Vos |
16:00 – 17:00 |
Theory: Filter technology
Post – versus in - column filters, types of microscopes, application flexibility versus resolution. |
Wim Busing |
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Thursday |
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09:00 – 12:00 |
Practical session: Repetition of different parts
- Morgagni
- CM 100
- T20 |
Vlada Philimonenko
Oldøich Benada
Matthijn Vos |
12:00 – 13:00 |
Lunch |
|
13:00 – 17:00 |
Practical session (continued)
- Morgagni
- CM 100
- T20 |
Vlada Philimonenko
Oldøich Benada
Matthijn Vos |
18:00 – ? |
Evening party - FEI sponsored |
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Friday |
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09:00 – 10:00 |
Theory: The best resolution and instrument requirements
The highest resolution in Life Sciences, best practice in biology. |
Matthijn Vos |
10:00 – 11:00 |
Theory: Sample preparation and cryo-electron microscopy; future prospects of TEM in biomedicine |
Pavel Hozák |
11:00 – 11:30 |
Short demonstration of sample preparation |
Vlada Philimonenko |
11:30 – 12:00 |
Evaluation of the workshop |
Pavel Hozák |
12:00 |
Lunch, departure |
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